7RSA
STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS
Summary for 7RSA
| Entry DOI | 10.2210/pdb7rsa/pdb |
| Descriptor | RIBONUCLEASE A, TERTIARY-BUTYL ALCOHOL (3 entities in total) |
| Functional Keywords | hydrolase (phosphoric diester) |
| Biological source | Bos taurus (cattle) |
| Total number of polymer chains | 1 |
| Total formula weight | 13782.45 |
| Authors | Wlodawer, A.,Gilliland, G.L. (deposition date: 1988-06-10, release date: 1988-10-09, Last modification date: 2024-05-22) |
| Primary citation | Wlodawer, A.,Svensson, L.A.,Sjolin, L.,Gilliland, G.L. Structure of phosphate-free ribonuclease A refined at 1.26 A. Biochemistry, 27:2705-2717, 1988 Cited by PubMed Abstract: The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme. PubMed: 3401445DOI: 10.1021/bi00408a010 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.26 Å) |
Structure validation
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