7RSA

STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	30.180, 38.400, 53.320
Unit cell angles	90.00, 105.85, 90.00

Refinement procedure

Resolution	10.000 * - 1.260
R-factor	0.15
RMSD bond length	0.024
RMSD bond angle	0.038 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.260 *
Rmerge	0.050 *
Total number of observations	129694 *
Number of reflections	25732 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.3 *		used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	50 (mg/ml)
2	1	1	sodium hydroxide	0.3 (M)
3	1	1	MPD	43 (%(v/v))