7RH2

IRF4 Transcription factor mutant -K59R

7RH2 の概要

• エントリーDOI: 10.2210/pdb7rh2/pdb
• 分子名称: ICSAT transcription factor, DNA (5'-D(*CP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*GP*AP*AP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*TP*TP*CP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*G)-3'), ... (4 entities in total)
• 機能のキーワード: irf4, cancer, homodimer complex, protein/dna interaction, transcription factor, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 75830.49

構造登録者

Williams, S.J.,Sundararaj, S.,Casarotto, M.G. (登録日: 2021-07-16, 公開日: 2022-05-25, 最終更新日: 2023-10-18)

主引用文献

Sundararaj, S.,Seneviratne, S.,Williams, S.J.,Enders, A.,Casarotto, M.G.
The molecular basis for the development of adult T-cell leukemia/lymphoma in patients with an IRF4 K59R mutation.
Protein Sci., 31:787-796, 2022

PubMed Abstract: Interferon regulatory factor 4 (IRF4) is an essential regulator in the development of many immune cells, including B- and T-cells and has been implicated directly in numerous hematological malignancies, including adult T-cell leukemia/lymphoma (ATLL). Recently, an activating mutation in the DNA-binding domain of IRF4 (IRF4 ) was found as a recurrent somatic mutation in ATLL patients. However, it remains unknown how this mutation gives rise to the observed oncogenic effect. To understand the mode of IRF4 -mediated gain of function in ATLL pathogenesis, we have determined the structural and affinity basis of IRF4 /DNA homodimer complex using X-ray crystallography and surface plasmon resonance. Our study shows that arginine substitution (R59) results in the reorientation of the side chain, enabling the guanidium group to interact with the phosphate backbone of the DNA helix. This markedly contrasts with the IRF4 wherein the K59 interacts exclusively with DNA bases. Further, the arginine mutation causes enhanced DNA bending, enabling the IRF4 to interact more robustly with known DNA targets, as evidenced by increased binding affinity of the protein-DNA complex. Together, we demonstrate how key structural features underpin the basis for this activating mutation, thereby providing a molecular rationale for IRF4 -mediated ATLL development.

PubMed: 34913532
DOI: 10.1002/pro.4260

実験手法

X-RAY DIFFRACTION (2.47 Å)