7REG

DfrA1 complexed with NADPH and 4'-chloro-3'-(4-(2,4-diamino-6-ethylpyrimidin-5-yl)but-3-yn-2-yl)-[1,1'-biphenyl]-4-carboxamide (UCP1228)

Summary for 7REG

• Entry DOI: 10.2210/pdb7reg/pdb
• Descriptor: Dihydrofolate reductase type 1, 4'-chloro-3'-[(2S)-4-(2,4-diamino-6-ethylpyrimidin-5-yl)but-3-yn-2-yl][1,1'-biphenyl]-4-carboxamide, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (5 entities in total)
• Functional Keywords: inhibitor, complex, antifolate, dhfr, oxidoreductase
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 37550.74

Authors

Lombardo, M.N.,Wright, D.L. (deposition date: 2021-07-12, release date: 2022-07-27, Last modification date: 2023-10-18)

• Primary citation: Krucinska, J.,Lombardo, M.N.,Erlandsen, H.,Estrada, A.,Si, D.,Viswanathan, K.,Wright, D.L.; Structure-guided functional studies of plasmid-encoded dihydrofolate reductases reveal a common mechanism of trimethoprim resistance in Gram-negative pathogens.; Commun Biol, 5:459-459, 2022; PubMed: 35562546; DOI: 10.1038/s42003-022-03384-y

Experimental method

X-RAY DIFFRACTION (1.77 Å)