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7RDQ

Cryo-EM structure of Thermus thermophilus reiterative transcription complex with 11nt oligo-G RNA

Summary for 7RDQ
Entry DOI10.2210/pdb7rdq/pdb
Related4g7h 7MLB 7MLJ
EMDB information24424
DescriptorDNA-directed RNA polymerase subunit alpha, ZINC ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
Functional Keywordsthermus thermophilus, transcription initiation, transcription
Biological sourceThermus thermophilus HB8
More
Total number of polymer chains9
Total formula weight451114.38
Authors
Liu, Y.,Ebright, R.H. (deposition date: 2021-07-10, release date: 2022-02-02, Last modification date: 2024-06-05)
Primary citationLiu, Y.,Yu, L.,Pukhrambam, C.,Winkelman, J.T.,Firlar, E.,Kaelber, J.T.,Zhang, Y.,Nickels, B.E.,Ebright, R.H.
Structural and mechanistic basis of reiterative transcription initiation.
Proc.Natl.Acad.Sci.USA, 119:-, 2022
Cited by
PubMed Abstract: Reiterative transcription initiation, observed at promoters that contain homopolymeric sequences at the transcription start site, generates RNA products having 5' sequences noncomplementary to the DNA template. Here, using crystallography and cryoelectron microscopy to define structures, protein-DNA photocrosslinking to map positions of RNAP leading and trailing edges relative to DNA, and single-molecule DNA nanomanipulation to assess RNA polymerase (RNAP)-dependent DNA unwinding, we show that RNA extension in reiterative transcription initiation 1) occurs without DNA scrunching; 2) involves a short, 2- to 3-bp, RNA-DNA hybrid; and 3) generates RNA that exits RNAP through the portal by which scrunched nontemplate-strand DNA exits RNAP in standard transcription initiation. The results establish that, whereas RNA extension in standard transcription initiation proceeds through a scrunching mechanism, RNA extension in reiterative transcription initiation proceeds through a slippage mechanism, with slipping of RNA relative to DNA within a short RNA-DNA hybrid, and with extrusion of RNA from RNAP through an alternative RNA exit.
PubMed: 35082149
DOI: 10.1073/pnas.2115746119
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3 Å)
Structure validation

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