7RDQ

Cryo-EM structure of Thermus thermophilus reiterative transcription complex with 11nt oligo-G RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	2	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Thermus thermophilus HB8	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	1	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Thermus thermophilus HB8	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	1	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermus thermophilus HB8	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	1	UniProt (Q8RQE7) Pfam (PF01192) In PDB	Thermus thermophilus HB8	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor SigA	polymer	423	48598.0	1	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Thermus thermophilus HB8
6	H	DNA (31-MER) nontemplate strand	polymer	33	10331.6	1		synthetic construct
7	G	DNA (31-MER) template strand	polymer	33	10100.5	1		synthetic construct
8	I	RNA (5'-R(P*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*G)-3')	polymer	11	3752.3	1		synthetic construct
9	B, D, F	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	450862.0
Non-Polymers*	Number of molecules	7
	Total formula weight	252.3
All*	Total formula weight	451114.4

*Water molecules are not included.