7QQD
Nuclear factor one X - NFIX in P21
Summary for 7QQD
| Entry DOI | 10.2210/pdb7qqd/pdb |
| Descriptor | Nuclear factor 1 X-type, NFI binding site (forward), NFI binding site (reverse), ... (6 entities in total) |
| Functional Keywords | transcription factor, nfi, nfix, nf-1, dna binding protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 4 |
| Total formula weight | 48834.95 |
| Authors | Lapi, M.,Chaves-Sanjuan, A.,Gourlay, L.J.,Tiberi, M.,Polentarutti, M.,Demitri, N.,Bais, G.,Nardini, M. (deposition date: 2022-01-07, release date: 2023-01-18, Last modification date: 2025-12-24) |
| Primary citation | Tiberi, M.,Lapi, M.,Gourlay, L.J.,Chaves-Sanjuan, A.,Polentarutti, M.,Demitri, N.,Cavinato, M.,Bonnet, D.M.V.,Taglietti, V.,Righetti, A.,Sala, R.,Cauteruccio, S.,Kumawat, A.,Russo, R.,Barbiroli, A.G.,Gnesutta, N.,Camilloni, C.,Bolognesi, M.,Messina, G.,Nardini, M. Structural basis of Nuclear Factor 1-X DNA recognition provides prototypic insight into the NFI family. Nat Commun, 16:10170-10170, 2025 Cited by PubMed Abstract: Nuclear Factor I (NFI) proteins are involved in adenovirus DNA replication and regulate gene transcription, stem cell proliferation, and differentiation. They play key roles in development, cancer, and congenital disorders. Within the NFI family, NFI-X is critical for neural stem cell biology, hematopoiesis, muscle development, muscular dystrophies, and oncogenesis. Here, we present the structural characterization of the NFI transcription factor NFI-X, both alone and bound to its consensus palindromic DNA site. Our analyses reveal a MH1-like fold within NFI-X DNA-binding domain (DBD) and identify crucial structural determinants for activity, such as a Zn²⁺ binding site, dimeric assembly, and DNA-binding specificity. Given the ~85% sequence identity within the NFI DBDs, our structural data are prototypic for the entire family, a NFI Rosetta Stone that allows decoding a wealth of biochemical and functional data and provides a precise target for drug design in a wider disease context. PubMed: 41261119DOI: 10.1038/s41467-025-65186-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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