7QDG

SARS-CoV-2 S protein S:A222V + S:D614G mutant 1-up

Summary for 7QDG

• Entry DOI: 10.2210/pdb7qdg/pdb
• EMDB information: 13916
• Descriptor: Spike glycoprotein,Fibritin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: sars-cov-2, s protein, s:a222v + s:d614g mutant, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2; More
• Total number of polymer chains: 3
• Total formula weight: 426990.81

Authors

Ginex, T.,Marco-Marin, C.,Wieczor, M.,Mata, C.P.,Krieger, J.,Lopez-Redondo, M.L.,Frances-Gomez, C.,Ruiz-Rodriguez, P.,Melero, R.,Sanchez-Sorzano, C.O.,Martinez, M.,Gougeard, N.,Forcada-Nadal, A.,Zamora-Caballero, S.,Gozalbo-Rovira, R.,Sanz-Frasquet, C.,Bravo, J.,Rubio, V.,Marina, A.,Geller, R.,Comas, I.,Gil, C.,Coscolla, M.,Orozco, M.,LLacer, J.L.,Carazo, J.M. (deposition date: 2021-11-27, release date: 2022-05-25, Last modification date: 2024-10-16)

Primary citation

Ginex, T.,Marco-Marin, C.,Wieczor, M.,Mata, C.P.,Krieger, J.,Ruiz-Rodriguez, P.,Lopez-Redondo, M.L.,Frances-Gomez, C.,Melero, R.,Sanchez-Sorzano, C.O.,Martinez, M.,Gougeard, N.,Forcada-Nadal, A.,Zamora-Caballero, S.,Gozalbo-Rovira, R.,Sanz-Frasquet, C.,Arranz, R.,Bravo, J.,Rubio, V.,Marina, A.,Geller, R.,Comas, I.,Gil, C.,Coscolla, M.,Orozco, M.,Llacer, J.L.,Carazo, J.M.
The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation.
Plos Pathog., 18:e1010631-e1010631, 2022

PubMed Abstract: The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serological, functional, structural and computational studies characterizing the impact of this mutation. Our results reveal that S:A222V promotes an increased RBD opening and slightly increases ACE2 binding as compared to the parent S:D614G clade. Finally, S:A222V does not reduce sera neutralization capacity, suggesting it does not affect vaccine effectiveness.

PubMed: 35816514
DOI: 10.1371/journal.ppat.1010631

Experimental method

ELECTRON MICROSCOPY (3.4 Å)