7QCU
Structure of the MUCIN-2 Cterminal domains partially deglycosylated.
Summary for 7QCU
| Entry DOI | 10.2210/pdb7qcu/pdb |
| Related | 7QCL |
| EMDB information | 13899 |
| Descriptor | Mucin-2, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total) |
| Functional Keywords | mucus, extracellular, net assemble, structural protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 180990.40 |
| Authors | Gallego, P.,Hansson, G.C. (deposition date: 2021-11-25, release date: 2023-03-08, Last modification date: 2024-11-20) |
| Primary citation | Gallego, P.,Garcia-Bonete, M.J.,Trillo-Muyo, S.,Recktenwald, C.V.,Johansson, M.E.V.,Hansson, G.C. The intestinal MUC2 mucin C-terminus is stabilized by an extra disulfide bond in comparison to von Willebrand factor and other gel-forming mucins. Nat Commun, 14:1969-1969, 2023 Cited by PubMed Abstract: The MUC2 mucin polymer is the main building unit of the intestinal mucus layers separating intestinal microbiota from the host epithelium. The MUC2 mucin is a large glycoprotein with a C-terminal domain similar to the MUC5AC and MUC5B mucins and the von Willebrand factor (VWF). A structural model of the C-terminal part of MUC2, MUC2-C, was generated by combining Cryo-electron microscopy, AlphaFold prediction, information of its glycosylation, and small angle X-ray scattering information. The globular VWD4 assembly in the N-terminal of MUC2-C is followed by 3.5 linear VWC domains that form an extended flexible structure before the C-terminal cystine-knot. All gel-forming mucins and VWF form tail-tail disulfide-bonded dimers in their C-terminal cystine-knot domain, but interestingly the MUC2 mucin has an extra stabilizing disulfide bond on the N-terminal side of the VWD4 domain, likely essential for a stable intestinal mucus barrier. PubMed: 37031240DOI: 10.1038/s41467-023-37666-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.25 Å) |
Structure validation
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