7PH3

AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position A60C

Summary for 7PH3

Entry DOI	10.2210/pdb7ph3/pdb
EMDB information	13405
Descriptor	ATP-dependent lipid A-core flippase, Nanobody Nb_MsbA#1, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (9 entities in total)
Functional Keywords	abc transporter, nanobody, amp-pnp, gd-dota, membrane protein
Biological source	Escherichia coli K-12 More
Total number of polymer chains	4
Total formula weight	161345.53
Authors	Parey, K.,Januliene, D.,Galazzo, L.,Meier, G.,Vecchis, D.,Striednig, B.,Hilbi, H.,Schaefer, L.V.,Kuprov, I.,Bordignon, E.,Seeger, M.A.,Moeller, A. (deposition date: 2021-08-16, release date: 2022-08-24, Last modification date: 2022-10-26)
Primary citation	Galazzo, L.,Meier, G.,Januliene, D.,Parey, K.,De Vecchis, D.,Striednig, B.,Hilbi, H.,Schafer, L.V.,Kuprov, I.,Moeller, A.,Bordignon, E.,Seeger, M.A. The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells. Sci Adv, 8:eabn6845-eabn6845, 2022 Cited by PubMed: 36223470 DOI: 10.1126/sciadv.abn6845 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.8 Å)

Structure validation