7PH3
AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position A60C
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | ATP-dependent lipid A-core flippase | polymer | 593 | 65760.9 | 2 | UniProt (P60752) Pfam (PF00664) Pfam (PF00005) In PDB | Escherichia coli K-12 | Lipid A export ATP-binding/permease protein MsbA,Lipid flippase |
2 | C, D | Nanobody Nb_MsbA#1 | polymer | 117 | 12441.0 | 2 | Vicugna pacos | ||
3 | A, B | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A, B | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 2 | Chemie (LMT) | |||
6 | A, B | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 2 | Chemie (3PE) | |||
7 | C, D | (1~{R},4~{R},11~{S},14~{S},19~{Z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione | non-polymer | 524.5 | 2 | Chemie (88T) | |||
8 | C, D | GADOLINIUM ATOM | non-polymer | 157.3 | 2 | Chemie (GD) | |||
9 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 582 (UniProt: P60752)
PDB | External Database | Details |
---|---|---|
Gly -10 | - | expression tag |
Pro -9 | - | expression tag |
Asp -8 | - | expression tag |
Glu -7 | - | expression tag |
Ala -6 | - | expression tag |
Glu -5 | - | expression tag |
Lys -4 | - | expression tag |
Leu -3 | - | expression tag |
Phe -2 | - | expression tag |
Asn -1 | - | expression tag |
Gln 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 156403.6 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 4941.9 | |
All* | Total formula weight | 161345.5 |