7PH2

Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position A60C

Summary for 7PH2

• Entry DOI: 10.2210/pdb7ph2/pdb
• EMDB information: 13404
• Descriptor: ATP-dependent lipid A-core flippase, Nb_MsbA#1, (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-4-[(3~{R})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{R})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{S},5~{S},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanylnonanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid, ... (5 entities in total)
• Functional Keywords: abc transporter, nanobody, gd-dota, membrane protein
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 4
• Total formula weight: 159831.51

Authors

Januliene, D.,Parey, K.,Galazzo, L.,Meier, G.,Vecchis, D.,Striednig, B.,Hilbi, H.,Schaefer, L.V.,Kuprov, I.,Bordignon, E.,Seeger, M.A.,Moeller, A. (deposition date: 2021-08-16, release date: 2022-08-24, Last modification date: 2022-10-26)

• Primary citation: Galazzo, L.,Meier, G.,Januliene, D.,Parey, K.,De Vecchis, D.,Striednig, B.,Hilbi, H.,Schafer, L.V.,Kuprov, I.,Moeller, A.,Bordignon, E.,Seeger, M.A.; The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells.; Sci Adv, 8:eabn6845-eabn6845, 2022; PubMed: 36223470; DOI: 10.1126/sciadv.abn6845

Experimental method

ELECTRON MICROSCOPY (3.7 Å)