7PGJ

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G

Summary for 7PGJ

Entry DOI	10.2210/pdb7pgj/pdb
Descriptor	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB, S-ADENOSYL-L-HOMOCYSTEINE, methyl (1R,2R,4S)-2-ethyl-7-methoxy-2,4,5-tris(oxidanyl)-6,11-bis(oxidanylidene)-3,4-dihydro-1H-tetracene-1-carboxylate, ... (4 entities in total)
Functional Keywords	carminomycin-4-o-methyltransferase variant; chimeragenesis; aclacinomycin 10-hydroxylase, tsukubarubicin 10-decarboxylase; biosynthetic protein, biosynthetic protein
Biological source	Streptomyces peucetius More
Total number of polymer chains	1
Total formula weight	40721.15
Authors	Dinis, P.,MetsaKetela, M. (deposition date: 2021-08-14, release date: 2022-08-24, Last modification date: 2024-02-07)
Primary citation	Dinis, P.,Tirkkonen, H.,Wandi, B.N.,Siitonen, V.,Niemi, J.,Grocholski, T.,Metsa-Ketela, M. Evolution-inspired engineering of anthracycline methyltransferases. Pnas Nexus, 2:pgad009-pgad009, 2023 Cited by PubMed: 36874276 DOI: 10.1093/pnasnexus/pgad009 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.13 Å)

Structure validation