7P6S

Crystal structure of the FimH-binding decoy module of human glycoprotein 2 (GP2) (crystal form II)

7P6S の概要

• エントリーDOI: 10.2210/pdb7p6s/pdb
• 関連するPDBエントリー: 7P6R 7P6T
• 分子名称: Isoform Alpha of Pancreatic secretory granule membrane major glycoprotein GP2, 2-acetamido-2-deoxy-beta-D-glucopyranose, pentane-1,5-diol, ... (4 entities in total)
• 機能のキーワード: extracellular matrix, glycoprotein, n-glycan, structural protein, decoy module, d10c domain, d8c sequence, pancreas, intestine, mucosal immunity, fimh, alphafold, antimicrobial protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18457.42

構造登録者

Stsiapanava, A.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Jovine, L. (登録日: 2021-07-17, 公開日: 2022-03-16, 最終更新日: 2024-05-01)

主引用文献

Stsiapanava, A.,Xu, C.,Nishio, S.,Han, L.,Yamakawa, N.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Wu, B.,Jovine, L.
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Nat.Struct.Mol.Biol., 29:190-193, 2022

PubMed Abstract: Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast nephropathy.

PubMed: 35273390
DOI: 10.1038/s41594-022-00729-3

実験手法

X-RAY DIFFRACTION (1.35 Å)