7P5Z

Structure of a DNA-loaded MCM double hexamer engaged with the Dbf4-dependent kinase

This is a non-PDB format compatible entry.

Summary for 7P5Z

• Entry DOI: 10.2210/pdb7p5z/pdb
• Related: 7P30
• EMDB information: 13176 13211
• Descriptor: DNA replication licensing factor MCM2, DNA (53-MER), ADENOSINE-5'-TRIPHOSPHATE, ... (14 entities in total)
• Functional Keywords: kinase, mcm2-7 helicase, nucleoprotein complex, dna replication, replication
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 16
• Total formula weight: 1401822.80

Authors

Greiwe, J.F.,Miller, T.C.R.,Martino, F.,Costa, A. (deposition date: 2021-07-15, release date: 2022-02-02, Last modification date: 2024-07-17)

Primary citation

Greiwe, J.F.,Miller, T.C.R.,Locke, J.,Martino, F.,Howell, S.,Schreiber, A.,Nans, A.,Diffley, J.F.X.,Costa, A.
Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase.
Nat.Struct.Mol.Biol., 29:10-20, 2022

PubMed Abstract: Loading of the eukaryotic replicative helicase onto replication origins involves two MCM hexamers forming a double hexamer (DH) around duplex DNA. During S phase, helicase activation requires MCM phosphorylation by Dbf4-dependent kinase (DDK), comprising Cdc7 and Dbf4. DDK selectively phosphorylates loaded DHs, but how such fidelity is achieved is unknown. Here, we determine the cryogenic electron microscopy structure of Saccharomyces cerevisiae DDK in the act of phosphorylating a DH. DDK docks onto one MCM ring and phosphorylates the opposed ring. Truncation of the Dbf4 docking domain abrogates DH phosphorylation, yet Cdc7 kinase activity is unaffected. Late origin firing is blocked in response to DNA damage via Dbf4 phosphorylation by the Rad53 checkpoint kinase. DDK phosphorylation by Rad53 impairs DH phosphorylation by blockage of DDK binding to DHs, and also interferes with the Cdc7 active site. Our results explain the structural basis and regulation of the selective phosphorylation of DNA-loaded MCM DHs, which supports bidirectional replication.

PubMed: 34963704
DOI: 10.1038/s41594-021-00698-z

Experimental method

ELECTRON MICROSCOPY (3.3 Å)