7P5Z
Structure of a DNA-loaded MCM double hexamer engaged with the Dbf4-dependent kinase
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, G (2, A) | DNA replication licensing factor MCM2 | polymer | 868 | 98911.5 | 2 | UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Minichromosome maintenance protein 2 |
| 2 | B, H (3, B) | DNA replication licensing factor MCM3 | polymer | 1006 | 111720.2 | 2 | UniProt (P24279) Pfam (PF00493) Pfam (PF17855) Pfam (PF22207) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Minichromosome maintenance protein 3 |
| 3 | C, I (4, C) | DNA replication licensing factor MCM4 | polymer | 933 | 105138.4 | 2 | UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Cell division control protein 54 |
| 4 | D, J (5, D) | Minichromosome maintenance protein 5 | polymer | 775 | 86505.7 | 2 | UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Cell division control protein 46 |
| 5 | E, K (6, E) | DNA replication licensing factor MCM6 | polymer | 1017 | 113110.2 | 2 | UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Minichromosome maintenance protein 6 |
| 6 | F, L (7, F) | DNA replication licensing factor MCM7 | polymer | 845 | 95049.9 | 2 | UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Cell division control protein 47,Minichromosome maintenance protein 7 |
| 7 | M (1) | Cell division control protein 7 | polymer | 507 | 58391.1 | 1 | UniProt (P06243) Pfam (PF00069) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 8 | N (G) | DDK kinase regulatory subunit DBF4 | polymer | 738 | 84503.3 | 1 | UniProt (P32325) Pfam (PF22437) Pfam (PF08630) Pfam (PF07535) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Dumbbell forming protein 4 |
| 9 | O (X) | DNA (53-MER) | polymer | 53 | 16326.4 | 1 | Saccharomyces cerevisiae S288C | ||
| 10 | P (Y) | DNA (53-MER) | polymer | 53 | 16335.5 | 1 | Saccharomyces cerevisiae S288C | ||
| 11 | FA, Q (A, 2) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 12 | BA, DA, GA, JA, NA... (7, A, B, D, F...) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) | |||
| 13 | AA, EA, HA, LA, OA... (6, 7, A, C, D...) | ZINC ION | non-polymer | 65.4 | 11 | Chemie (ZN) | |||
| 14 | CA, IA, KA, MA, RA... (7, B, C, D, F...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 8 | Chemie (ADP) |
Sequence modifications
3, B: 1 - 971 (UniProt: P24279)
G: 1 - 704 (UniProt: P32325)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Lys -33 | - | expression tag |
| Arg -32 | - | expression tag |
| Arg -31 | - | expression tag |
| Trp -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Lys -28 | - | expression tag |
| Asn -27 | - | expression tag |
| Phe -26 | - | expression tag |
| Ile -25 | - | expression tag |
| Ala -24 | - | expression tag |
| Val -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Ala -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Asn -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Phe -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Ile -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Glu 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Lys -33 | - | expression tag |
| Arg -32 | - | expression tag |
| Arg -31 | - | expression tag |
| Trp -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Lys -28 | - | expression tag |
| Asn -27 | - | expression tag |
| Phe -26 | - | expression tag |
| Ile -25 | - | expression tag |
| Ala -24 | - | expression tag |
| Val -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Ala -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Asn -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Phe -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Ile -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Glu 0 | - | expression tag |
| Ala 10 | Arg 10 | conflict |
| Ala 13 | Leu 13 | conflict |
| Ala 62 | Arg 62 | conflict |
| Ala 65 | Leu 65 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 1396428.3 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 5394.5 | |
| All* | Total formula weight | 1401822.8 |
