7P5X

Mycobacterial RNAP with transcriptional activator PafBC

This is a non-PDB format compatible entry.

Summary for 7P5X

• Entry DOI: 10.2210/pdb7p5x/pdb
• EMDB information: 13205
• Descriptor: DNA-directed RNA polymerase subunit alpha, Transcriptional regulator-like protein, ZINC ION, ... (11 entities in total)
• Functional Keywords: transcription, activator, sigma adaption, rna polymerase, rnap, pafb, pafc, pafbc, mycobacterium, smegmatis
• Biological source: Mycolicibacterium smegmatis MC2 155; More
• Total number of polymer chains: 11
• Total formula weight: 545245.01

Authors

Mueller, A.U.,Kummer, E.,Schilling, C.M.,Ban, N.,Weber-Ban, E. (deposition date: 2021-07-15, release date: 2021-12-22, Last modification date: 2024-07-17)

Primary citation

Muller, A.U.,Kummer, E.,Schilling, C.M.,Ban, N.,Weber-Ban, E.
Transcriptional control of mycobacterial DNA damage response by sigma adaptation.
Sci Adv, 7:eabl4064-eabl4064, 2021

PubMed Abstract: Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo–electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical −10 and −35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term “sigma adaptation.” PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the −35 but featuring the canonical −10 and a PafBC-specific −26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria.

PubMed: 34851662
DOI: 10.1126/sciadv.abl4064

Experimental method

ELECTRON MICROSCOPY (3.2 Å)