7P5D

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) mutant (serine 317 and 319 to alanine)

Summary for 7P5D

• Entry DOI: 10.2210/pdb7p5d/pdb
• Related: 6ELC 7P56 7P57 7P59 7P5A 7P5B
• Descriptor: Variant surface glycoprotein, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: variant surface glycoprotein, coat, trypanosoma brucei, o-glycosylation, o-glycans, immune evasion, african trypanosome, antigenic variation, post-translational modification, vsg, parasite, sleeping sickness, membrane protein
• Biological source: Trypanosoma brucei brucei
• Total number of polymer chains: 1
• Total formula weight: 54975.36

Authors

Gkeka, A.,Aresta-Branco, F.,Stebbins, C.E.,Papavasiliou, F.N. (deposition date: 2021-07-14, release date: 2022-07-27, Last modification date: 2024-10-23)

Primary citation

Gkeka, A.,Aresta-Branco, F.,Triller, G.,Vlachou, E.P.,van Straaten, M.,Lilic, M.,Olinares, P.D.B.,Perez, K.,Chait, B.T.,Blatnik, R.,Ruppert, T.,Verdi, J.P.,Stebbins, C.E.,Papavasiliou, F.N.
Immunodominant surface epitopes power immune evasion in the African trypanosome.
Cell Rep, 42:112262-112262, 2023

PubMed Abstract: The African trypanosome survives the immune response of its mammalian host by antigenic variation of its major surface antigen (the variant surface glycoprotein or VSG). Here we describe the antibody repertoires elicited by different VSGs. We show that the repertoires are highly restricted and are directed predominantly to distinct epitopes on the surface of the VSGs. They are also highly discriminatory; minor alterations within these exposed epitopes confer antigenically distinct properties to these VSGs and elicit different repertoires. We propose that the patterned and repetitive nature of the VSG coat focuses host immunity to a restricted set of immunodominant epitopes per VSG, eliciting a highly stereotyped response, minimizing cross-reactivity between different VSGs and facilitating prolonged immune evasion through epitope variation.

PubMed: 36943866
DOI: 10.1016/j.celrep.2023.112262

Experimental method

X-RAY DIFFRACTION (1.42 Å)