7P59

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) with two O-linked post-translational modifications

7P59 の概要

• エントリーDOI: 10.2210/pdb7p59/pdb
• 関連するPDBエントリー: 7P56 7P57 7P5A 7P5B 7P5D
• 分子名称: Variant surface glycoprotein, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: variant surface glycoprotein, coat, trypanosoma brucei, o-glycosylation, o-glycans, immune evasion, african trypanosome, antigenic variation, post-translational modification, vsg, parasite, sleeping sickness, membrane protein
• 由来する生物種: Trypanosoma brucei brucei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55367.67

構造登録者

Gkeka, A.,Arest-Branco, F.,Stebbins, C.E.,Papavasiliou, F.N. (登録日: 2021-07-14, 公開日: 2022-07-27, 最終更新日: 2024-11-13)

主引用文献

Gkeka, A.,Aresta-Branco, F.,Triller, G.,Vlachou, E.P.,van Straaten, M.,Lilic, M.,Olinares, P.D.B.,Perez, K.,Chait, B.T.,Blatnik, R.,Ruppert, T.,Verdi, J.P.,Stebbins, C.E.,Papavasiliou, F.N.
Immunodominant surface epitopes power immune evasion in the African trypanosome.
Cell Rep, 42:112262-112262, 2023

PubMed Abstract: The African trypanosome survives the immune response of its mammalian host by antigenic variation of its major surface antigen (the variant surface glycoprotein or VSG). Here we describe the antibody repertoires elicited by different VSGs. We show that the repertoires are highly restricted and are directed predominantly to distinct epitopes on the surface of the VSGs. They are also highly discriminatory; minor alterations within these exposed epitopes confer antigenically distinct properties to these VSGs and elicit different repertoires. We propose that the patterned and repetitive nature of the VSG coat focuses host immunity to a restricted set of immunodominant epitopes per VSG, eliciting a highly stereotyped response, minimizing cross-reactivity between different VSGs and facilitating prolonged immune evasion through epitope variation.

PubMed: 36943866
DOI: 10.1016/j.celrep.2023.112262

実験手法

X-RAY DIFFRACTION (1.27 Å)