7OQY

Cryo-EM structure of the cellular negative regulator TFS4 bound to the archaeal RNA polymerase

7OQY の概要

• エントリーDOI: 10.2210/pdb7oqy/pdb
• 関連するPDBエントリー: 7ok0 7oq4
• EMDBエントリー: 12960 13026 13034
• 分子名称: DNA-directed RNA polymerase subunit A', DNA-directed RNA polymerase subunit L, DNA-directed RNA polymerase subunit N, ... (17 entities in total)
• 機能のキーワード: negative regulator, inhibitor, archaea, sso tfs4, trancript cleavage factor, transcription
• 由来する生物種: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 416527.14

構造登録者

Pilotto, S.,Fouqueau, T.,Lukoyanova, N.,Sheppard, C.,Lucas-Staat, S.,Diaz-Santin, L.M.,Matelska, D.,Prangishvili, D.,Cheung, A.C.M.,Werner, F. (登録日: 2021-06-04, 公開日: 2021-08-25, 最終更新日: 2024-07-17)

主引用文献

Pilotto, S.,Fouqueau, T.,Lukoyanova, N.,Sheppard, C.,Lucas-Staat, S.,Diaz-Santin, L.M.,Matelska, D.,Prangishvili, D.,Cheung, A.C.M.,Werner, F.
Structural basis of RNA polymerase inhibition by viral and host factors.
Nat Commun, 12:5523-5523, 2021

PubMed Abstract: RNA polymerase inhibition plays an important role in the regulation of transcription in response to environmental changes and in the virus-host relationship. Here we present the high-resolution structures of two such RNAP-inhibitor complexes that provide the structural bases underlying RNAP inhibition in archaea. The Acidianus two-tailed virus encodes the RIP factor that binds inside the DNA-binding channel of RNAP, inhibiting transcription by occlusion of binding sites for nucleic acid and the transcription initiation factor TFB. Infection with the Sulfolobus Turreted Icosahedral Virus induces the expression of the host factor TFS4, which binds in the RNAP funnel similarly to eukaryotic transcript cleavage factors. However, TFS4 allosterically induces a widening of the DNA-binding channel which disrupts trigger loop and bridge helix motifs. Importantly, the conformational changes induced by TFS4 are closely related to inactivated states of RNAP in other domains of life indicating a deep evolutionary conservation of allosteric RNAP inhibition.

PubMed: 34535646
DOI: 10.1038/s41467-021-25666-5

実験手法

ELECTRON MICROSCOPY (2.61 Å)