7OFQ
The archaellum of Methanocaldococcus villosus
Summary for 7OFQ
| Entry DOI | 10.2210/pdb7ofq/pdb |
| EMDB information | 12875 |
| Descriptor | Archaellin, CALCIUM ION, UNKNOWN LIGAND, ... (4 entities in total) |
| Functional Keywords | archaellum, archaellin, methanocaldococcus villosus, cryoem, helical reconstruction, protein fibril |
| Biological source | Methanocaldococcus villosus More |
| Total number of polymer chains | 45 |
| Total formula weight | 999762.07 |
| Authors | Isupov, M.,Gambelli, L.,Daum, B. (deposition date: 2021-05-05, release date: 2022-01-19, Last modification date: 2025-10-01) |
| Primary citation | Gambelli, L.,Isupov, M.N.,Conners, R.,McLaren, M.,Bellack, A.,Gold, V.,Rachel, R.,Daum, B. An archaellum filament composed of two alternating subunits. Nat Commun, 13:710-710, 2022 Cited by PubMed Abstract: Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament's flexibility. PubMed: 35132062DOI: 10.1038/s41467-022-28337-1 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.08 Å) |
Structure validation
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