7OFQ
The archaellum of Methanocaldococcus villosus
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.08 |
Number of Particle | 929165 |
Software | EMAN2,RELION,cryoSPARC,REFMAC,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |