7NHK

LsaA, an antibiotic resistance ABCF, in complex with 70S ribosome from Enterococcus faecalis

This is a non-PDB format compatible entry.

Summary for 7NHK

• Entry DOI: 10.2210/pdb7nhk/pdb
• EMDB information: 12331
• Descriptor: ABC-F type ribosomal protection protein Lsa(A), 5S rRNA, 50S ribosomal protein L2, ... (58 entities in total)
• Functional Keywords: antibiotic resistance element, ribosomal protein, lsaa, abcf, target protection, antibiotic resistance, ribosome
• Biological source: Enterococcus faecalis; More
• Total number of polymer chains: 53
• Total formula weight: 2232137.36

Authors

Crowe-McAuliffe, C.,Kasari, M.,Hauryliuk, V.H.,Wilson, D.N. (deposition date: 2021-02-10, release date: 2021-05-05, Last modification date: 2024-07-10)

Primary citation

Crowe-McAuliffe, C.,Murina, V.,Turnbull, K.J.,Kasari, M.,Mohamad, M.,Polte, C.,Takada, H.,Vaitkevicius, K.,Johansson, J.,Ignatova, Z.,Atkinson, G.C.,O'Neill, A.J.,Hauryliuk, V.,Wilson, D.N.
Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
Nat Commun, 12:3577-3577, 2021

PubMed Abstract: Target protection proteins confer resistance to the host organism by directly binding to the antibiotic target. One class of such proteins are the antibiotic resistance (ARE) ATP-binding cassette (ABC) proteins of the F-subtype (ARE-ABCFs), which are widely distributed throughout Gram-positive bacteria and bind the ribosome to alleviate translational inhibition from antibiotics that target the large ribosomal subunit. Here, we present single-particle cryo-EM structures of ARE-ABCF-ribosome complexes from three Gram-positive pathogens: Enterococcus faecalis LsaA, Staphylococcus haemolyticus VgaA and Listeria monocytogenes VgaL. Supported by extensive mutagenesis analysis, these structures enable a general model for antibiotic resistance mediated by these ARE-ABCFs to be proposed. In this model, ABCF binding to the antibiotic-stalled ribosome mediates antibiotic release via mechanistically diverse long-range conformational relays that converge on a few conserved ribosomal RNA nucleotides located at the peptidyltransferase center. These insights are important for the future development of antibiotics that overcome such target protection resistance mechanisms.

PubMed: 34117249
DOI: 10.1038/s41467-021-23753-1

Experimental method

ELECTRON MICROSCOPY (2.9 Å)