7N78

Cryo-EM structure of ATP13A2 in the E2-Pi state

Summary for 7N78

• Entry DOI: 10.2210/pdb7n78/pdb
• EMDB information: 24212 24213 24214 24215 24216 24217 24218 24219 24220 24221 24222 24223
• Descriptor: Isoform 3 of Polyamine-transporting ATPase 13A2, PHOSPHATE ION, MAGNESIUM ION, ... (8 entities in total)
• Functional Keywords: p-type atpase, p5b-atpase, polyamine transporter, transport protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 133338.40

Authors

Sim, S.I.,Park, E. (deposition date: 2021-06-09, release date: 2021-11-10, Last modification date: 2024-06-05)

Primary citation

Sim, S.I.,von Bulow, S.,Hummer, G.,Park, E.
Structural basis of polyamine transport by human ATP13A2 (PARK9).
Mol.Cell, 81:4635-4649.e8, 2021

PubMed Abstract: Polyamines are small, organic polycations that are ubiquitous and essential to all forms of life. Currently, how polyamines are transported across membranes is not understood. Recent studies have suggested that ATP13A2 and its close homologs, collectively known as P5B-ATPases, are polyamine transporters at endo-/lysosomes. Loss-of-function mutations of ATP13A2 in humans cause hereditary early-onset Parkinson's disease. To understand the polyamine transport mechanism of ATP13A2, we determined high-resolution cryoelectron microscopy (cryo-EM) structures of human ATP13A2 in five distinct conformational intermediates, which together, represent a near-complete transport cycle of ATP13A2. The structural basis of the polyamine specificity was revealed by an endogenous polyamine molecule bound to a narrow, elongated cavity within the transmembrane domain. The structures show an atypical transport path for a water-soluble substrate, in which polyamines may exit within the cytosolic leaflet of the membrane. Our study provides important mechanistic insights into polyamine transport and a framework to understand the functions and mechanisms of P5B-ATPases.

PubMed: 34715013
DOI: 10.1016/j.molcel.2021.08.017

Experimental method

ELECTRON MICROSCOPY (3 Å)