7ML4
RNA polymerase II initially transcribing complex (ITC)
Summary for 7ML4
| Entry DOI | 10.2210/pdb7ml4/pdb |
| EMDB information | 23904 23905 23906 23907 23908 |
| Descriptor | Transcription initiation factor IIF subunit alpha, DNA-directed RNA polymerases I,II,and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC3, ... (34 entities in total) |
| Functional Keywords | pic, tfiih, transcription, itc, rna polymerase ii |
| Biological source | Saccharomyces cerevisiae (baker's yeast) More |
| Total number of polymer chains | 31 |
| Total formula weight | 1333462.04 |
| Authors | Yang, C.,Fujiwara, R.,Kim, H.J.,Gorbea Colon, J.J.,Steimle, S.,Garcia, B.A.,Murakami, K. (deposition date: 2021-04-27, release date: 2022-02-02, Last modification date: 2024-12-25) |
| Primary citation | Yang, C.,Fujiwara, R.,Kim, H.J.,Basnet, P.,Zhu, Y.,Gorbea Colon, J.J.,Steimle, S.,Garcia, B.A.,Kaplan, C.D.,Murakami, K. Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II. Mol.Cell, 82:660-676.e9, 2022 Cited by PubMed Abstract: Previous structural studies of the initiation-elongation transition of RNA polymerase II (pol II) transcription have relied on the use of synthetic oligonucleotides, often artificially discontinuous to capture pol II in the initiating state. Here, we report multiple structures of initiation complexes converted de novo from a 33-subunit yeast pre-initiation complex (PIC) through catalytic activities and subsequently stalled at different template positions. We determine that PICs in the initially transcribing complex (ITC) can synthesize a transcript of ∼26 nucleotides before transitioning to an elongation complex (EC) as determined by the loss of general transcription factors (GTFs). Unexpectedly, transition to an EC was greatly accelerated when an ITC encountered a downstream EC stalled at promoter proximal regions and resulted in a collided head-to-end dimeric EC complex. Our structural analysis reveals a dynamic state of TFIIH, the largest of GTFs, in PIC/ITC with distinct functional consequences at multiple steps on the pathway to elongation. PubMed: 35051353DOI: 10.1016/j.molcel.2021.12.020 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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