7LYT
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and Phosphorothioate-DNA
Summary for 7LYT
Entry DOI | 10.2210/pdb7lyt/pdb |
Related | 7LYS |
EMDB information | 23600 23601 |
Descriptor | CasPhi, crRNA, TS-DNA, ... (6 entities in total) |
Functional Keywords | crispr, casphi, cas12j, nuclease, r-loop, crrna, pam, rnp, complex, viral protein-rna-dna, viral protein-rna-dna complex, viral protein/rna/dna |
Biological source | Biggievirus Mos11 (Biggievirus) More |
Total number of polymer chains | 4 |
Total formula weight | 127411.01 |
Authors | Pausch, P.,Soczek, K.,Nogales, E.,Doudna, J. (deposition date: 2021-03-08, release date: 2021-08-04, Last modification date: 2024-05-29) |
Primary citation | Pausch, P.,Soczek, K.M.,Herbst, D.A.,Tsuchida, C.A.,Al-Shayeb, B.,Banfield, J.F.,Nogales, E.,Doudna, J.A. DNA interference states of the hypercompact CRISPR-Cas Phi effector. Nat.Struct.Mol.Biol., 28:652-661, 2021 Cited by PubMed: 34381246DOI: 10.1038/s41594-021-00632-3 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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