7LYT

Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and Phosphorothioate-DNA

Summary for 7LYT

• Entry DOI: 10.2210/pdb7lyt/pdb
• Related: 7LYS
• EMDB information: 23600 23601
• Descriptor: CasPhi, crRNA, TS-DNA, ... (6 entities in total)
• Functional Keywords: crispr, casphi, cas12j, nuclease, r-loop, crrna, pam, rnp, complex, viral protein-rna-dna, viral protein-rna-dna complex, viral protein/rna/dna
• Biological source: Biggievirus Mos11 (Biggievirus); More
• Total number of polymer chains: 4
• Total formula weight: 127411.01

Authors

Pausch, P.,Soczek, K.,Nogales, E.,Doudna, J. (deposition date: 2021-03-08, release date: 2021-08-04, Last modification date: 2024-05-29)

• Primary citation: Pausch, P.,Soczek, K.M.,Herbst, D.A.,Tsuchida, C.A.,Al-Shayeb, B.,Banfield, J.F.,Nogales, E.,Doudna, J.A.; DNA interference states of the hypercompact CRISPR-Cas Phi effector.; Nat.Struct.Mol.Biol., 28:652-661, 2021; PubMed: 34381246; DOI: 10.1038/s41594-021-00632-3

Experimental method

ELECTRON MICROSCOPY (2.9 Å)