7L7W
Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E mutant
Summary for 7L7W
| Entry DOI | 10.2210/pdb7l7w/pdb |
| Descriptor | Probable disease resistance protein At5g66900, NICKEL (II) ION (3 entities in total) |
| Functional Keywords | 4-helix bundle, cell death, membrane pore, calcium channel, immune system |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) |
| Total number of polymer chains | 1 |
| Total formula weight | 16034.87 |
| Authors | Walton, W.G.,Wan, L.,Lietzan, A.D.,Redinbo, M.R.,Dangl, J.L. (deposition date: 2020-12-30, release date: 2021-06-16, Last modification date: 2023-10-18) |
| Primary citation | Jacob, P.,Kim, N.H.,Wu, F.,El-Kasmi, F.,Chi, Y.,Walton, W.G.,Furzer, O.J.,Lietzan, A.D.,Sunil, S.,Kempthorn, K.,Redinbo, M.R.,Pei, Z.M.,Wan, L.,Dangl, J.L. Plant "helper" immune receptors are Ca 2+ -permeable nonselective cation channels. Science, 373:420-425, 2021 Cited by PubMed Abstract: Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In , a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca) influx in plant and human cells. NRG1.1-dependent Ca influx and cell death were sensitive to Ca channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that helper NLRs form Ca-permeable cation channels to directly regulate cytoplasmic Ca levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly. PubMed: 34140391DOI: 10.1126/science.abg7917 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
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