7L7W

Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0300
Spacegroup name	H 3
Unit cell lengths	114.257, 114.257, 59.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.130 - 2.550
R-factor	0.1971
Rwork	0.193
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7l7v
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.590
High resolution limit [Å]	2.550	6.910	2.550
Rmerge	0.105	0.059	0.908
Rmeas	0.111	0.062	1.014
Rpim	0.036	0.020	0.437
Total number of observations	78469
Number of reflections	9220	471	338
<I/σ(I)>	7.3
Completeness [%]	96.2	100	72.7
Redundancy	8.5	9.7	3.9
CC(1/2)		0.998	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES: NaOH (pH 7.5), 0.3 M Magnesium Formate