7KHN

NicA2 variant N462Y/W427Y in complex with (S)-nicotine

Summary for 7KHN

• Entry DOI: 10.2210/pdb7khn/pdb
• Related: 7KHO
• Descriptor: Amine oxidase, FLAVIN-ADENINE DINUCLEOTIDE, (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE, ... (4 entities in total)
• Functional Keywords: flavoenzyme, oxidoreductase, nicotine, rossmann-core fold, flavoprotein
• Biological source: Pseudomonas putida (strain S16)
• Total number of polymer chains: 1
• Total formula weight: 54801.44

Authors

Tararina, M.A.,Allen, K.N. (deposition date: 2020-10-21, release date: 2021-02-03, Last modification date: 2023-10-18)

• Primary citation: Tararina, M.A.,Dam, K.K.,Dhingra, M.,Janda, K.D.,Palfey, B.A.,Allen, K.N.; Fast Kinetics Reveals Rate-Limiting Oxidation and the Role of the Aromatic Cage in the Mechanism of the Nicotine-Degrading Enzyme NicA2.; Biochemistry, 60:259-273, 2021; PubMed: 33464876; DOI: 10.1021/acs.biochem.0c00855

Experimental method

X-RAY DIFFRACTION (2.31 Å)