7JTA
Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium
Summary for 7JTA
| Entry DOI | 10.2210/pdb7jta/pdb |
| Descriptor | NTF2-like nuclease/anti-CRISPR, NITRATE ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total) |
| Functional Keywords | anti-crispr, nuclease, phage protein, cas9, unknown function |
| Biological source | Clostridia bacterium |
| Total number of polymer chains | 2 |
| Total formula weight | 13340.87 |
| Authors | Werther, R.,Forsberg, K.J.,Stoddard, B.L. (deposition date: 2020-08-17, release date: 2021-09-22, Last modification date: 2024-10-30) |
| Primary citation | Forsberg, K.J.,Schmidtke, D.T.,Werther, R.,Uribe, R.V.,Hausman, D.,Sommer, M.O.A.,Stoddard, B.L.,Kaiser, B.K.,Malik, H.S. The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity. Plos Biol., 19:e3001428-e3001428, 2021 Cited by PubMed Abstract: To overcome CRISPR-Cas defense systems, many phages and mobile genetic elements (MGEs) encode CRISPR-Cas inhibitors called anti-CRISPRs (Acrs). Nearly all characterized Acrs directly bind Cas proteins to inactivate CRISPR immunity. Here, using functional metagenomic selection, we describe AcrIIA22, an unconventional Acr found in hypervariable genomic regions of clostridial bacteria and their prophages from human gut microbiomes. AcrIIA22 does not bind strongly to SpyCas9 but nonetheless potently inhibits its activity against plasmids. To gain insight into its mechanism, we obtained an X-ray crystal structure of AcrIIA22, which revealed homology to PC4-like nucleic acid-binding proteins. Based on mutational analyses and functional assays, we deduced that acrIIA22 encodes a DNA nickase that relieves torsional stress in supercoiled plasmids. This may render them less susceptible to SpyCas9, which uses free energy from negative supercoils to form stable R-loops. Modifying DNA topology may provide an additional route to CRISPR-Cas resistance in phages and MGEs. PubMed: 34644300DOI: 10.1371/journal.pbio.3001428 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.801 Å) |
Structure validation
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