7JTA

Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium

Summary for 7JTA

Entry DOI	10.2210/pdb7jta/pdb
Descriptor	NTF2-like nuclease/anti-CRISPR, NITRATE ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
Functional Keywords	anti-crispr, nuclease, phage protein, cas9, unknown function
Biological source	Clostridia bacterium
Total number of polymer chains	2
Total formula weight	13340.87
Authors	Werther, R.,Forsberg, K.J.,Stoddard, B.L. (deposition date: 2020-08-17, release date: 2021-09-22, Last modification date: 2024-04-03)
Primary citation	Forsberg, K.J.,Schmidtke, D.T.,Werther, R.,Uribe, R.V.,Hausman, D.,Sommer, M.O.A.,Stoddard, B.L.,Kaiser, B.K.,Malik, H.S. The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity. Plos Biol., 19:e3001428-e3001428, 2021 Cited by PubMed: 34644300 DOI: 10.1371/journal.pbio.3001428 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.801 Å)

Structure validation