7JTA
Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2018-08-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.02111 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 128.561, 128.561, 128.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.854 - 2.801 |
| R-factor | 0.2247 |
| Rwork | 0.222 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Robetta model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.106 | 0.084 | 0.906 |
| Rmeas | 0.112 | 0.090 | 0.953 |
| Rpim | 0.034 | 0.029 | 0.289 |
| Total number of observations | 96848 | ||
| Number of reflections | 9344 | 987 | 920 |
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 98.7 | 92.1 | 100 |
| Redundancy | 10.4 | 9.4 | 10.7 |
| CC(1/2) | 0.995 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 40% MPD, 0.2M ammonium nitrate, 10mM MgCl2 |
