7JJK

Crystal structure of SOX30

Summary for 7JJK

• Entry DOI: 10.2210/pdb7jjk/pdb
• Descriptor: Transcription factor SOX-30 (2 entities in total)
• Functional Keywords: high mobility group, sox30, sox, transcription factor, hmg box, transcription
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 10502.07

Authors

Ghafoori, S.M.,Forwood, J.K. (deposition date: 2020-07-27, release date: 2020-08-19, Last modification date: 2025-09-10)

Primary citation

Ghafoori, S.M.,Sethi, A.,Petersen, G.F.,Tanipour, M.H.,Gooley, P.R.,Forwood, J.K.
RNA Binding Properties of SOX Family Members.
Cells, 13:-, 2024

PubMed Abstract: SOX proteins are a family of transcription factors (TFs) that play critical functions in sex determination, neurogenesis, and chondrocyte differentiation, as well as cardiac, vascular, and lymphatic development. There are 20 SOX family members in humans, each sharing a 79-residue L-shaped high mobility group (HMG)-box domain that is responsible for DNA binding. SOX2 was recently shown to interact with long non-coding RNA and large-intergenic non-coding RNA to regulate embryonic stem cell and neuronal differentiation. The RNA binding region was shown to reside within the HMG-box domain; however, the structural details of this binding remain unclear. Here, we show that all SOX family members, except group H, interact with RNA. Our mutational experiments demonstrate that the disordered C-terminal region of the HMG-box domain plays an important role in RNA binding. Further, by determining a high-resolution structure of the HMG-box domain of the group H family member SOX30, we show that despite differences in RNA binding ability, SOX30 shares a very similar secondary structure with other SOX protein HMG-box domains. Together, our study provides insight into the interaction of SOX TFs with RNA.

PubMed: 39056784
DOI: 10.3390/cells13141202

Experimental method

X-RAY DIFFRACTION (1.4 Å)