7ELG

LC3B modificated with a covalent probe

Summary for 7ELG

• Entry DOI: 10.2210/pdb7elg/pdb
• Descriptor: Microtubule-associated proteins 1A/1B light chain 3B, 2-methylidene-5-thiophen-2-yl-cyclohexane-1,3-dione, SULFATE ION, ... (4 entities in total)
• Functional Keywords: inhibitor, complex, protein binding
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 14781.89

Authors

Fan, S.,Wan, W. (deposition date: 2021-04-10, release date: 2021-10-13, Last modification date: 2024-10-16)

Primary citation

Fan, S.,Yue, L.,Wan, W.,Zhang, Y.,Zhang, B.,Otomo, C.,Li, Q.,Lin, T.,Hu, J.,Xu, P.,Zhu, M.,Tao, H.,Chen, Z.,Li, L.,Ding, H.,Yao, Z.,Lu, J.,Wen, Y.,Zhang, N.,Tan, M.,Chen, K.,Xie, Y.,Otomo, T.,Zhou, B.,Jiang, H.,Dang, Y.,Luo, C.
Inhibition of Autophagy by a Small Molecule through Covalent Modification of the LC3 Protein.
Angew.Chem.Int.Ed.Engl., 60:26105-26114, 2021

PubMed Abstract: The autophagic ubiquitin-like protein LC3 functions through interactions with LC3-interaction regions (LIRs) of other autophagy proteins, including autophagy receptors, which stands out as a promising protein-protein interaction (PPI) target for the intervention of autophagy. Post-translational modifications like acetylation of Lys49 on the LIR-interacting surface could disrupt the interaction, offering an opportunity to design covalent small molecules interfering with the interface. Through screening covalent compounds, we discovered a small molecule modulator of LC3A/B that covalently modifies LC3A/B protein at Lys49. Activity-based protein profiling (ABPP) based evaluations reveal that a derivative molecule DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells. DC-LC3in-D5 compromises LC3B lipidation in vitro and in HeLa cells, leading to deficiency in the formation of autophagic structures and autophagic substrate degradation. DC-LC3in-D5 could serve as a powerful tool for autophagy research as well as for therapeutic interventions.

PubMed: 34590387
DOI: 10.1002/anie.202109464

Experimental method

X-RAY DIFFRACTION (1.599 Å)