7ELG
LC3B modificated with a covalent probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.108, 54.766, 60.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.402 - 1.599 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.402 | 1.656 |
| High resolution limit [Å] | 1.599 | 1.599 |
| Rmerge | 0.037 | 0.469 |
| Number of reflections | 16025 | 1562 |
| <I/σ(I)> | 36.72 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.0M ammonium sulfate, 0.1M tri-sodium citrate (pH 5.6), 0.2M potassium sodium tartrate |
