7EHL

Cryo-EM structure of human ABCB8 transporter in nucleotide binding state

7EHL の概要

• エントリーDOI: 10.2210/pdb7ehl/pdb
• EMDBエントリー: 31142
• 分子名称: Mitochondrial potassium channel ATP-binding subunit, CHOLESTEROL, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: abc transporter, membrane protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 149864.37

構造登録者

Li, S.J.,Yang, X.,Shen, Y.Q. (登録日: 2021-03-29, 公開日: 2021-05-05, 最終更新日: 2025-04-09)

主引用文献

Li, S.,Ren, Y.,Lu, X.,Shen, Y.,Yang, X.
Cryo-EM structure of human ABCB8 transporter in nucleotide binding state.
Biochem.Biophys.Res.Commun., 557:187-191, 2021

PubMed Abstract: Human ATP-binding cassette transporter 8 of subfamily B (hABCB8) is an ABC transporter that located in the inner membrane of mitochondria. The ABCB8 is involved in the maturation of Fe-S and protects the heart from oxidative stress. Here, we present the cryo-EM structure of human ABCB8 binding with AMPPNP in inward-facing conformation with resolution of 4.1 Å. hABCB8 shows an open-inward conformation when ATP is bound. Unexpectedly, cholesterol molecules were identified in the transmembrane domain of hABCB8. Our results provide structural basis for the transport mechanism of the ABC transporter in mitochondria.

PubMed: 33872987
DOI: 10.1016/j.bbrc.2021.04.007

実験手法

ELECTRON MICROSCOPY (4.1 Å)