7CP1

Crystal structure of isocitrate lyase in complex with succinate and itaconate

Summary for 7CP1

• Entry DOI: 10.2210/pdb7cp1/pdb
• Descriptor: Isocitrate lyase, SUCCINIC ACID, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: glyoxylate cycle, isocitrate lyase, succinate, itaconate, mycobacterium tuberculosis, lyase
• Biological source: Mycobacterium tuberculosis H37Rv
• Total number of polymer chains: 2
• Total formula weight: 96707.86

Authors

Kwon, S.,Park, H.H. (deposition date: 2020-08-05, release date: 2021-05-19, Last modification date: 2023-11-29)

Primary citation

Kwon, S.,Chun, H.L.,Ha, H.J.,Lee, S.Y.,Park, H.H.
Heterogeneous multimeric structure of isocitrate lyase in complex with succinate and itaconate provides novel insights into its inhibitory mechanism.
Plos One, 16:e0251067-e0251067, 2021

PubMed Abstract: During the glyoxylate cycle, isocitrate lyases (ICLs) catalyze the lysis of isocitrate to glyoxylate and succinate. Itaconate has been reported to inhibit an ICL from Mycobacterium tuberculosis (tbICL). To elucidate the molecular mechanism of ICL inhibition, we determined the crystal structure of tbICL in complex with itaconate. Unexpectedly, succinate and itaconate were found to bind to the respective active sites in the dimeric form of tbICL. Our structure revealed the active site architecture as an open form, although the substrate and inhibitor were bound to the active sites. Our findings provide novel insights into the conformation of tbICL upon its binding to a substrate or inhibitor, along with molecular details of the inhibitory mechanism of itaconate.

PubMed: 33951112
DOI: 10.1371/journal.pone.0251067

Experimental method

X-RAY DIFFRACTION (2.58 Å)