7CK4
Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2
Summary for 7CK4
| Entry DOI | 10.2210/pdb7ck4/pdb |
| Descriptor | Heat shock protein (1 entity in total) |
| Functional Keywords | small heat shock protein, oligomers, nanocage, chaperone activity., chaperone |
| Biological source | Synechococcus phage S-ShM2 |
| Total number of polymer chains | 6 |
| Total formula weight | 116956.28 |
| Authors | Biswas, S.,Suguna, K. (deposition date: 2020-07-15, release date: 2021-07-21, Last modification date: 2023-11-29) |
| Primary citation | Biswas, S.,Garg, P.,Dutta, S.,Suguna, K. Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries. Sci Rep, 11:21023-21023, 2021 Cited by PubMed Abstract: The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 Å. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 Å. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. PubMed: 34697325DOI: 10.1038/s41598-021-00172-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (7 Å) |
Structure validation
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