7CK4
Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | I 4 |
| Unit cell lengths | 126.146, 126.146, 152.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.650 - 7.000 |
| R-factor | 0.35063 |
| Rwork | 0.346 |
| R-free | 0.43350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zja |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 7.830 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.164 | 1.530 |
| Number of reflections | 1915 | 557 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.5 | |
| CC(1/2) | 0.994 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2M Lithium sulphate monohydrate, 0.1M Tris, 25% PEG 3350 |
