7CK4

Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9536
Spacegroup name	I 4
Unit cell lengths	126.146, 126.146, 152.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 7.000
R-factor	0.35063
Rwork	0.346
R-free	0.43350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zja
RMSD bond length	0.006
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	7.830
High resolution limit [Å]	7.000	7.000
Rmerge	0.164	1.530
Number of reflections	1915	557
<I/σ(I)>	9.5
Completeness [%]	99.4	100
Redundancy	6.5
CC(1/2)	0.994	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.2M Lithium sulphate monohydrate, 0.1M Tris, 25% PEG 3350