7C9O
Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)
Summary for 7C9O
| Entry DOI | 10.2210/pdb7c9o/pdb |
| Descriptor | Zinc finger protein HD1, Nuclear transcription factor Y subunit B-11, Nuclear transcription factor Y subunit C-2, ... (6 entities in total) |
| Functional Keywords | photoperiodic flowering, cct family, nf-cct complex, specific dna recognition, transcription |
| Biological source | Oryza sativa Japonica Group (Rice) More |
| Total number of polymer chains | 5 |
| Total formula weight | 46192.43 |
| Authors | |
| Primary citation | Shen, C.,Liu, H.,Guan, Z.,Yan, J.,Zheng, T.,Yan, W.,Wu, C.,Zhang, Q.,Yin, P.,Xing, Y. Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering. Plant Cell, 32:3469-3484, 2020 Cited by PubMed Abstract: CONSTANS, CONSTANS-LIKE, and TIMING OF CAB EXPRESSION1 (CCT) domain-containing proteins are a large family unique to plants. They transcriptionally regulate photoperiodic flowering, circadian rhythms, vernalization, and other related processes. Through their CCT domains, CONSTANS and HEADING DATE1 (HD1) coordinate with the NUCLEAR FACTOR Y (NF-Y) B/C dimer to specifically target a conserved 'CCACA' motif within the promoters of their target genes. However, the mechanism underlying DNA recognition by the CCT domain remains unclear. Here we determined the crystal structures of the rice () NF-YB/YC dimer and the florigen gene ()-bound HD1/NF-YB/YC trimer with resolutions of 2.0 Å and 2.55 Å, respectively. The CCT domain of HD1 displays an elongated structure containing two α-helices and two loops, tethering to the NF-YB/YC dimer. Helix α2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide insight into the network between the CCT proteins and NF-Y subunits, but also offer potential approaches for improving productivity and global adaptability of crops by manipulating florigen expression. PubMed: 32843433DOI: 10.1105/tpc.20.00067 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
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