7C08

Crystal structure of S34Y mutant of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.

7C08 の概要

• エントリーDOI: 10.2210/pdb7c08/pdb
• 分子名称: Splicing factor U2AF 23 kDa subunit, Splicing factor U2AF 59 kDa subunit, RNA (5'-R(*U*UP*AP*GP*GP*U)-3'), ... (4 entities in total)
• 機能のキーワード: pre-mrna, splicing, intron, 3' splice site, zinc-finger, splicing-rna complex, splicing/rna
• 由来する生物種: Schizosaccharomyces pombe 972h- (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 314956.79

構造登録者

Yoshida, H.,Park, S.Y.,Urano, T.,Obayashi, E. (登録日: 2020-04-30, 公開日: 2020-09-30, 最終更新日: 2023-11-29)

主引用文献

Yoshida, H.,Park, S.Y.,Sakashita, G.,Nariai, Y.,Kuwasako, K.,Muto, Y.,Urano, T.,Obayashi, E.
Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.
Nat Commun, 11:4744-4744, 2020

PubMed Abstract: The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3´ splice site, which includes an essential AG dinucleotide. Even a single amino acid mutation of U2AF1 can cause serious disease such as certain cancers or myelodysplastic syndromes. Here, we describe the first crystal structures of wild-type and pathogenic mutant U2AF1 complexed with target RNA, revealing the mechanism of 3´ splice site selection, and how aberrant splicing results from clinically important mutations. Unexpected features of this mechanism may assist the future development of new treatments against diseases caused by splicing errors.

PubMed: 32958768
DOI: 10.1038/s41467-020-18559-6

実験手法

X-RAY DIFFRACTION (3.35 Å)