7BWN

Crystal Structure of a Designed Protein Heterocatenane

Summary for 7BWN

• Entry DOI: 10.2210/pdb7bwn/pdb
• Descriptor: Chimera of Green fluorescent protein and p53dim, Cellular tumor antigen p53 (3 entities in total)
• Functional Keywords: heterocatenane, recombination
• Biological source: Aequorea victoria (Jellyfish); More
• Total number of polymer chains: 16
• Total formula weight: 297092.54

Authors

Liu, Y.J.,Duan, Z.L.,Fang, J.,Zhang, F.,Xiao, J.Y.,Zhang, W.B. (deposition date: 2020-04-15, release date: 2020-06-17, Last modification date: 2023-11-29)

Primary citation

Liu, Y.,Duan, Z.,Fang, J.,Zhang, F.,Xiao, J.,Zhang, W.B.
Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane.
Angew.Chem.Int.Ed.Engl., 59:16122-16127, 2020

PubMed Abstract: Herein, we report the biosynthesis of protein heterocatenanes using a programmed sequence of multiple post-translational processing events including intramolecular chain entanglement, in situ backbone cleavage, and spontaneous cyclization. The approach is general, autonomous, and can obviate the need for any additional enzymes. The catenane topology was convincingly proven using a combination of SDS-PAGE, LC-MS, size exclusion chromatography, controlled proteolytic digestion, and protein crystallography. The X-ray crystal structure clearly shows two mechanically interlocked protein rings with intact folded domains. It opens new avenues in the nascent field of protein-topology engineering.

PubMed: 32506656
DOI: 10.1002/anie.202005490

Experimental method

X-RAY DIFFRACTION (2.396 Å)