7BWN

Crystal Structure of a Designed Protein Heterocatenane

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (F, A, C, E, H...)	Chimera of Green fluorescent protein and p53dim	polymer	280	31618.5	8	UniProt (A0A059PIQ0) Pfam (PF01353) Pfam (PF07710) UniProt (by SIFTS) (P42212) UniProt (by SIFTS) (P04637)	Aequorea victoria (Jellyfish)
2	B, D, F, H, J... (L, B, D, G, I...)	Cellular tumor antigen p53	polymer	46	5518.0	8	UniProt (P04637) Pfam (PF07710)	Homo sapiens (Human)	Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
3	AA, BA, CA, DA, EA... (J, K, M, N, O...)	water	water		18.0	565	Chemie (HOH)

Sequence modifications

F, A, C, E, H, J, M, O: 1 - 238 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	Arg 2	engineered mutation
Arg 30	Ser 30	engineered mutation
Ser 72	Ala 72	engineered mutation
Arg 80	Gln 80	engineered mutation
Val 206	Ala 206	engineered mutation

F, A, C, E, H, J, M, O: 239 - 280 (PDB: 7BWN)
L, B, D, G, I, K, N, P: 326 - 356 (UniProt: P04637)

PDB	External Database	Details
Gly 316	-	expression tag
Gly 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
Glu 324	-	expression tag
Leu 325	-	expression tag
Glu 340	Met 340	engineered mutation
Lys 344	Leu 344	engineered mutation
Thr 357	-	expression tag
Ser 358	-	expression tag
Cys 359	-	expression tag
Phe 360	-	expression tag
Asn 361	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	297092.5
All*	Total formula weight	297092.5

*Water molecules are not included.