7BVZ

Crystal structure of MreB5 of Spiroplasma citri bound to ADP

Summary for 7BVZ

• Entry DOI: 10.2210/pdb7bvz/pdb
• Descriptor: Cell shape determining protein MreB, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: cytoskeleton protein, atpase, protein fibril
• Biological source: Spiroplasma citri
• Total number of polymer chains: 1
• Total formula weight: 40160.37

Authors

Pande, V.,Bagde, S.R.,Gayathri, P. (deposition date: 2020-04-12, release date: 2020-10-14, Last modification date: 2023-11-29)

Primary citation

Harne, S.,Duret, S.,Pande, V.,Bapat, M.,Beven, L.,Gayathri, P.
MreB5 Is a Determinant of Rod-to-Helical Transition in the Cell-Wall-less Bacterium Spiroplasma.
Curr.Biol., 30:4753-, 2020

PubMed Abstract: In most rod-shaped bacteria, the spatial coordination of cell wall synthesis machinery by MreBs is the main theme for shape determination and maintenance in cell-walled bacteria [1-9]. However, how rod or spiral shapes are achieved and maintained in cell-wall-less bacteria is currently unknown. Spiroplasma, a helical Mollicute that lacks cell wall synthesis genes, encodes five MreB paralogs and a unique cytoskeletal protein fibril [10, 11]. Here, we show that MreB5, one of the five MreB paralogs, contributes to cell elongation and is essential for the transition from rod-to-helical shape in Spiroplasma. Comparative genomic and proteomic characterization of a helical and motile wild-type Spiroplasma strain and a non-helical, non-motile natural variant helped delineate the specific roles of MreB5. Moreover, complementation of the non-helical strain with MreB5 restored its helical shape and motility by a kink-based mechanism described for Spiroplasma [12]. Earlier studies had proposed that length changes in fibril filaments are responsible for the change in handedness of the helical cell and kink propagation during motility [13]. Through structural and biochemical characterization, we identify that MreB5 exists as antiparallel double protofilaments that interact with fibril and the membrane, and thus potentially assists in kink propagation. In summary, our study provides direct experimental evidence for MreB in maintaining cell length, helical shape, and motility-revealing the role of MreB in sculpting the cell in the absence of a cell wall.

PubMed: 32976813
DOI: 10.1016/j.cub.2020.08.093

Experimental method

X-RAY DIFFRACTION (2.3 Å)