7BVZ

Crystal structure of MreB5 of Spiroplasma citri bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-03-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	37.950, 41.150, 56.360
Unit cell angles	82.34, 74.01, 80.42

Refinement procedure

Resolution	36.142 - 2.300
R-factor	0.2303
Rwork	0.228
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czi
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.120	0.630
Rpim	0.110	0.520
Number of reflections	12584	1233
<I/σ(I)>	6.1	1.5
Completeness [%]	87.9	86.2
Redundancy	2.1	2.1
CC(1/2)	0.980	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	0.15M Na-K phosphate. 16% PEG 3350, pH 7.8, 2mM ADP, 2mM MgCl2