7BUV

Eucommia ulmoides TPT3, crystal form 2

Summary for 7BUV

• Entry DOI: 10.2210/pdb7buv/pdb
• Descriptor: FPS3 (1 entity in total)
• Functional Keywords: prenyltransferase, polyisoprene, plant protein
• Biological source: Eucommia ulmoides (Hardy rubber tree)
• Total number of polymer chains: 7
• Total formula weight: 295326.26

Authors

Kajiura, H.,Yoshizawa, T.,Tokumoto, Y.,Suzuki, N.,Takeno, S.,Takeno, K.J.,Yamashita, T.,Tanaka, S.,Kaneko, Y.,Fujiyama, K.,Matsumura, H.,Nakazawa, Y. (deposition date: 2020-04-08, release date: 2021-02-17, Last modification date: 2023-11-29)

Primary citation

Kajiura, H.,Yoshizawa, T.,Tokumoto, Y.,Suzuki, N.,Takeno, S.,Takeno, K.J.,Yamashita, T.,Tanaka, S.I.,Kaneko, Y.,Fujiyama, K.,Matsumura, H.,Nakazawa, Y.
Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis.
Commun Biol, 4:215-215, 2021

PubMed Abstract: Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms-EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants.

PubMed: 33594248
DOI: 10.1038/s42003-021-01739-5

Experimental method

X-RAY DIFFRACTION (3.3 Å)