7AY1

Cryo-EM structure of USP1-UAF1 bound to mono-ubiquitinated FANCD2, and FANCI

7AY1 の概要

• エントリーDOI: 10.2210/pdb7ay1/pdb
• EMDBエントリー: 11934
• 分子名称: Fanconi anemia group I protein, Fanconi anemia group D2 protein, Ubiquitin-60S ribosomal protein L40, ... (7 entities in total)
• 機能のキーワード: deubiquitination, specificity, dna repair, fanconi anemia, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 501070.06

構造登録者

Rennie, M.L.,Arkinson, C.,Walden, H. (登録日: 2020-11-10, 公開日: 2021-03-24, 最終更新日: 2024-10-23)

主引用文献

Rennie, M.L.,Arkinson, C.,Chaugule, V.K.,Toth, R.,Walden, H.
Structural basis of FANCD2 deubiquitination by USP1-UAF1.
Nat.Struct.Mol.Biol., 28:356-364, 2021

PubMed Abstract: Ubiquitin-specific protease 1 (USP1) acts together with the cofactor UAF1 during DNA repair processes to specifically remove monoubiquitin signals. One substrate of the USP1-UAF1 complex is the monoubiquitinated FANCI-FANCD2 heterodimer, which is involved in the repair of DNA interstrand crosslinks via the Fanconi anemia pathway. Here we determine structures of human USP1-UAF1 with and without ubiquitin and bound to monoubiquitinated FANCI-FANCD2. The crystal structures of USP1-UAF1 reveal plasticity in USP1 and key differences to USP12-UAF1 and USP46-UAF1, two related proteases. A cryo-EM reconstruction of USP1-UAF1 in complex with monoubiquitinated FANCI-FANCD2 highlights a highly orchestrated deubiquitination process, with USP1-UAF1 driving conformational changes in the substrate. An extensive interface between UAF1 and FANCI, confirmed by mutagenesis and biochemical assays, provides a molecular explanation for the requirement of both proteins, despite neither being directly involved in catalysis. Overall, our data provide molecular details of USP1-UAF1 regulation and substrate recognition.

PubMed: 33795880
DOI: 10.1038/s41594-021-00576-8

実験手法

ELECTRON MICROSCOPY (3.7 Å)