7ANK

Crystal structure of sarcomeric protein FATZ-1 (d91-FATZ-1 construct) in complex with half dimer of alpha-actinin-2

7ANK の概要

• エントリーDOI: 10.2210/pdb7ank/pdb
• 関連するPDBエントリー: 7A8T 7A8U
• 分子名称: Alpha-actinin-2, Myozenin-1 (3 entities in total)
• 機能のキーワード: z-disk complex, f-actin crosslinking protein, scaffolding protein, fuzzy complex, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 128694.74

構造登録者

Sponga, A.,Arolas, J.L.,Rodriguez Chamorro, A.,Mlynek, G.,Hollerl, E.,Schreiner, C.,Pedron, M.,Kostan, J.,Ribeiro, E.A.,Djinovic-Carugo, K. (登録日: 2020-10-12, 公開日: 2021-06-30, 最終更新日: 2024-01-31)

主引用文献

Sponga, A.,Arolas, J.L.,Schwarz, T.C.,Jeffries, C.M.,Rodriguez Chamorro, A.,Kostan, J.,Ghisleni, A.,Drepper, F.,Polyansky, A.,De Almeida Ribeiro, E.,Pedron, M.,Zawadzka-Kazimierczuk, A.,Mlynek, G.,Peterbauer, T.,Doto, P.,Schreiner, C.,Hollerl, E.,Mateos, B.,Geist, L.,Faulkner, G.,Kozminski, W.,Svergun, D.I.,Warscheid, B.,Zagrovic, B.,Gautel, M.,Konrat, R.,Djinovic-Carugo, K.
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with alpha-actinin.
Sci Adv, 7:-, 2021

PubMed Abstract: In sarcomeres, α-actinin cross-links actin filaments and anchors them to the Z-disk. FATZ (filamin-, α-actinin-, and telethonin-binding protein of the Z-disk) proteins interact with α-actinin and other core Z-disk proteins, contributing to myofibril assembly and maintenance. Here, we report the first structure and its cellular validation of α-actinin-2 in complex with a Z-disk partner, FATZ-1, which is best described as a conformational ensemble. We show that FATZ-1 forms a tight fuzzy complex with α-actinin-2 and propose an interaction mechanism via main molecular recognition elements and secondary binding sites. The obtained integrative model reveals a polar architecture of the complex which, in combination with FATZ-1 multivalent scaffold function, might organize interaction partners and stabilize α-actinin-2 preferential orientation in Z-disk. Last, we uncover FATZ-1 ability to phase-separate and form biomolecular condensates with α-actinin-2, raising the question whether FATZ proteins can create an interaction hub for Z-disk proteins through membraneless compartmentalization during myofibrillogenesis.

PubMed: 34049882
DOI: 10.1126/sciadv.abg7653

実験手法

X-RAY DIFFRACTION (3.204 Å)