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7A9W

Structure of yeast Rmd9p in complex with 20nt target RNA

Summary for 7A9W
Entry DOI10.2210/pdb7a9w/pdb
DescriptorProtein RMD9, mitochondrial, RNA (5'-R(*AP*UP*AP*AP*AP*AP*UP*AP*AP*CP*AP*UP*UP*CP*UP*U)-3'), CHLORIDE ION, ... (4 entities in total)
Functional Keywordsmitochondria, translation, transcription, degradation, ribosome, rna binding protein
Biological sourceSaccharomyces cerevisiae S288C
More
Total number of polymer chains2
Total formula weight76606.34
Authors
Hillen, H.S.,Markov, D.A.,Ireneusz, W.D.,Hofmann, K.B.,Cowan, A.T.,Jones, J.L.,Temiakov, D.,Cramer, P.,Anikin, M. (deposition date: 2020-09-02, release date: 2021-04-07, Last modification date: 2021-05-05)
Primary citationHillen, H.S.,Markov, D.A.,Wojtas, I.D.,Hofmann, K.B.,Lidschreiber, M.,Cowan, A.T.,Jones, J.L.,Temiakov, D.,Cramer, P.,Anikin, M.
The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3'-UTRs of yeast mitochondrial mRNAs.
Proc.Natl.Acad.Sci.USA, 118:-, 2021
Cited by
PubMed Abstract: Stabilization of messenger RNA is an important step in posttranscriptional gene regulation. In the nucleus and cytoplasm of eukaryotic cells it is generally achieved by 5' capping and 3' polyadenylation, whereas additional mechanisms exist in bacteria and organelles. The mitochondrial mRNAs in the yeast comprise a dodecamer sequence element that confers RNA stability and 3'-end processing via an unknown mechanism. Here, we isolated the protein that binds the dodecamer and identified it as Rmd9, a factor that is known to stabilize yeast mitochondrial RNA. We show that Rmd9 associates with mRNA around dodecamer elements in vivo and that recombinant Rmd9 specifically binds the element in vitro. The crystal structure of Rmd9 bound to its dodecamer target reveals that Rmd9 belongs to the family of pentatricopeptide (PPR) proteins and uses a previously unobserved mode of specific RNA recognition. Rmd9 protects RNA from degradation by the mitochondrial 3'-exoribonuclease complex mtEXO in vitro, indicating that recognition and binding of the dodecamer element by Rmd9 confers stability to yeast mitochondrial mRNAs.
PubMed: 33876744
DOI: 10.1073/pnas.2009329118
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.55 Å)
Structure validation

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