7A9W
Structure of yeast Rmd9p in complex with 20nt target RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 106.670, 106.670, 128.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.240 - 2.550 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.23330 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.378 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | XDS |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.087 | 3.001 |
| Number of reflections | 53148 | 3965 |
| <I/σ(I)> | 18.16 | 0.87 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.8 | 10.6 |
| CC(1/2) | 1.000 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293.15 | Na-Cacodylate, NaCl, LiSO4, CaAcetate, MgCl2, PEG8000 |
