7WSM

Cryo-EM structure of human glucose transporter GLUT4 bound to cytochalasin B in lipid nanodiscs

Summary for 7WSM

• Entry DOI: 10.2210/pdb7wsm/pdb
• EMDB information: 32760
• Descriptor: Solute carrier family 2, facilitated glucose transporter member 4, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Cytochalasin B (3 entities in total)
• Functional Keywords: glucose transporter, glut4, diabetes, cytochalasin b, transport protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 57012.21

Authors

Yuan, Y.,Kong, F.,Xu, H.,Zhu, A.,Yan, N.,Yan, C. (deposition date: 2022-01-30, release date: 2022-05-18, Last modification date: 2024-11-20)

Primary citation

Yuan, Y.,Kong, F.,Xu, H.,Zhu, A.,Yan, N.,Yan, C.
Cryo-EM structure of human glucose transporter GLUT4.
Nat Commun, 13:2671-2671, 2022

PubMed Abstract: GLUT4 is the primary glucose transporter in adipose and skeletal muscle tissues. Its cellular trafficking is regulated by insulin signaling. Failed or reduced plasma membrane localization of GLUT4 is associated with diabetes. Here, we report the cryo-EM structures of human GLUT4 bound to a small molecule inhibitor cytochalasin B (CCB) at resolutions of 3.3 Å in both detergent micelles and lipid nanodiscs. CCB-bound GLUT4 exhibits an inward-open conformation. Despite the nearly identical conformation of the transmembrane domain to GLUT1, the cryo-EM structure reveals an extracellular glycosylation site and an intracellular helix that is invisible in the crystal structure of GLUT1. The structural study presented here lays the foundation for further mechanistic investigation of the modulation of GLUT4 trafficking. Our methods for cryo-EM analysis of GLUT4 will also facilitate structural determination of many other small size solute carriers.

PubMed: 35562357
DOI: 10.1038/s41467-022-30235-5

Experimental method

ELECTRON MICROSCOPY (3.25 Å)